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Superintendent
David Davis
21262 Genoa Road
Linneus, MO 64653
Phone: 660 895-5121
FAX: 660 895=5122
Email:
DavisDK@missouri.edu
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October 1, 2002
Forage Systems Update
Vol 11, No. 4
Grass-Legume Mixtures:
Annual Variability of Red Clover and Birdsfoot Trefoil
Jim Gerrish
For the past twenty years, most of the pastures at FSRC have been
managed as grass-legume mixtures with very little annual input of
nitrogen fertilizer. When we make recommendations to producers to
switch from N-based to legume based pastures two common concerns are
the relative yield of grass-legume mixtures compared to N-fertilized
pastures and what is the reliability of legumes from year to year. A
third less commonly asked. but of equal importance, question is the
relative cost of production. In several previous issues we have
featured different aspects of our pasture diversity study. We have
completed five years of grazing on these plots and can begin to
evaluate some of these common questions.
While the study includes 16 pasture mixtures of different
complexity, this report will only be looking at the simplest
mixtures to evaluate the role and behavior of red clover and
birdsfoot trefoil in tall fescue pastures. The four treatments being
compared are tall fescue + 80 lb N/acre (TF+N), tall fescue with no
seeded legume or N (TF-0), tall fescue + red clover (TF+RC), and
tall fescue + birdsfoot trefoil (TF+BFT). Grass was seeded in
September, 1994, and the legumes were frost seeded in March, 1995.
There has been no additional interseeding since the original
planting. Any legume regeneration has come by way of natural
reseeding. The pastures are maintained at a medium soil test level
with target pH of 6, P1 of 20 lb/acre, and exchangeable K of 200
lb/acre. Soil tests taken in 2001 did not meet these targets and 0-
60-250 was applied in September, 2001.
In each year from 1998 through 2002, individual plots were grazed
whenever a particular plot reached 8 to 10-in. mean sward height.
Plots were grazed with six to eight steers for four to seven hours
to remove approximately 50% of the forage biomass. Basal cover of
species composition was determined by step-point method with 100
points recorded for each plot. In 1998 stand measurements were made
prior to each grazing event while in subsequent years measurements
were made approximately every 4 weeks regardless of regrowth stage
from April through October.
Trend lines for red clover and birdsfoot trefoil cover were
determined using linear regression as first, second, and third order
models with best-fit model reported . Variability was calculated as
standard deviation divided by the sample mean. Variability is simply
a measurement of year to year change in presence of that species in
the mixture. Low variability means greater predictability of pasture
performance and, hence, greater reliability across a range of
weather conditions. We looked at these two legumes both as seeded
and invader species.
Results and discussion: Variations in pasture yield among the four
mixtures occurred each year, but not in a consistent manner. Year
to year variations in forage yield can be attributed to rainfall
variations, but also to species composition. Forage yield was
significantly lower in 1999 and 2000 when growing season rainfall
was substantially lower than normal. The TF+N pasture had
significantly higher forage yield two out of five years compared to
the other mixtures. In 1998, 1999, and 2000, forage yield of TF+BFT
was not significantly different from TF+N. (Figure 1). In four out
of five years, TF+0 yielded significantly less than all other
mixtures. It is inevitable that there will be pasture yield
variations from year to year. We are interested in understanding why
variations occur and what we can do to minimize those variations.
Table 1. Monthly rainfall, seasonal total, mean yield of all forage
mixtures, and forage dry matter yield per inch of rainfall for the
period 1998-2002.
| Year | Apr | May | Jun | Jul | Aug | Sep | 6-mo total | Annual Yield | DMY/inch rainfall |
|   | ------------------------------inches------------------------------ | (lb/acre) | (lb/inch) |
| 1998 | 4.90 | 2.19 | 10.74 | 3.90 | 2.13 | 6.97 | 30.83 | 8724 | 283 |
| 1999 | 5.86 | 5.87 | 2.58 | 1.55 | 1.10 | 5.88 | 22.84 | 6985 | 306 |
| 2000 | 0.85 | 2.39 | 6.67 | 4.50 | 5.41 | 2.53 | 22.35 | 6729 | 301 |
| 2001 | 4.14 | 7.62 | 5.97 | 4.04 | 4.04 | 4.26 | 30.07 | 7968 | 265 |
| 2002 | 4.25 | 12.86 | 1.24 | 4.28 | 7.97 | 1.34 | 31.94 | 8029 | 251 |
|   |
| 30-yr norm | 3.51 | 5.49 | 4.34 | 4.92 | 4.34 | 3.75 | 26.35 | 7687 | 281 |
 | | Figure 1. Five-year mean yield for four pasture mixtures with hashed bars indicating mean of all four mixtures for that year | Just by looking at Figure 1, it is apparent that some types of
pasture are more affected by weather than others. First glance
suggests that TF+RC exhibited more yield variance than any other
mixture. We will use the term ‘variability' to describe the
likelihood and range of annual yield variance within a particular
mixture. The calculated forage yield variability are below:
| TF+N | = | 11% ab |
| TF+0 | = | 14% b |
| TF+BFT | = | 8% a |
| TF+RC | = | 21% c |
This value describes how much annual variation can be expected in
forage yield of a particular mixture. It is a mathematical
description of what Figure 1 suggests visually. The greatest amount
of annual variance occurs in tall fescue-red clover mixtures and the
least occurs in tall fescue-birdsfoot trefoil mixtures. The
letter(s) following each value indicate which variability indices
are significantly different.
These results indicate that either tall fescue with N fertilization
or tall fescue with birdsfoot trefoil are the most reliable pastures
across a range in rainfall conditions. Birdsfoot trefoil is plagued
with a wide range of root and crown diseases in the humid Midwestern
environment so it may be surprising to some that it is such a stable
mixture. The key is in its ability to naturally reseed itself. The
higher rate of variability in red clover is a reflection of its
lesser ability for natural reseeding without extended rest periods.
In this study with a target grazing height of 8 to 10 inches, red
clover was not adequately rested to allow reseeding. So the key
factor is stand persistence, not individual plant persistence.
We also looked at variations in the species composition of the
mixtures over the five year period.  | | Figure 2. The trend line for birdsfoot trefoil indicates only minor changes over time. | While stand density is not a
direct link to pasture yield, it is a relative indicator of species
contribution to forage yield. In the first two years of this study
we did hand separation of individual species to determine yield
contribution, but limited resources did not allow us to continue
collecting this (tedious) data. Stand density does vary within
season depending on growth habit of the species in question, weather
conditions, and stage of growth during a grazing event. The figures
below show the individual data points for each mixture at each
sampling and a fitted regression line to indicate the trend and
direction of change in composition.
The relatively low variance in birdsfoot trefoil stand density is
consistent with the forage yield data reported above. One of the
advantages frequently cited for birdsfoot trefoil is that it can
tolerate both excessively dry and excessively wet soils and seasons.
These are characteristics that would help minimize seasonal and site
variations. The drier conditions experienced in 1999 and 2000 may
have reduced the competitiveness of companion species allowing
trefoil to flourish.
 | | Figure 3. The trend for red clover exhibits a much wider annual variation in stand density. |
The annual variance in red clover stand density was much more
pronounced than was variance in trefoil (Figure 3). This is, again,
very consistent with the forage yield data. Individual red clover
plants are generally considered to live only two or three years
making reseeding a necessary part of stand survival. In this study,
no annual interseeding was made after the original frost seeding in
1995 so any plants present during the course of this study were the
result of natural regeneration. Average stand density of red clover
was lower than that of birdsfoot trefoil although peak stand density
were similar for the two legumes.
Another piece of data that was collected in some, but not all years
were legume seedling counts taken in May. In 1999 very few
seedlings were counted while in 2000 a relatively high number of
seedlings were found. In 1998 the mean rest period for TF+RC
pastures was 31 days while in the much drier 1999 season, mean rest
period was 46 days. The opportunity for reseeding was much greater
in 1999 than it was in 1998. Factors like these combined with
seasonal weather variations tend to make red clover more cyclic in
its presence and yield contribution in pasture.
 | | Figure 4. The trend line for birdsfoot trefoil indicates a more aggressive invader habit than red clover. |
Thus far we have considered these two legumes from the perspective
of seeded species. We are frequently interested in the ability of
desirable plant species to disperse across the farm and colonize new
pastures. Legume seed has the capacity to pass through the rumen as
hard seed and be redistributed across the landscape via manure. It
is a very common sight in the spring to see dung pats from the
previous autumn or winter sprouting legume seedlings. One of our
concerns is how many of those seedlings survive and become
established plants in a pasture. This is the relative colonizing
property of a species.
The TF+0 treatment did not have either red clover or trefoil seeded
in the pasture but has been invaded over time by both species
(Figure 4). Comparing the trend lines for the two species, we see
that birdsfoot trefoil has a much stronger colonizing ability than
does red clover. Red clover continued to exhibit its cyclic habit
while trefoil showed a steady increase in stand density.
The practical way to reduce the cyclic nature of red clover is to
overseed on a regular basis. Recommendations range from annually to
every three years. Annual overseeding with about four pounds of red
clover can make red clover much more consistent and reliable.
Avoiding overgrazing and allowing periodic rest of birdsfoot trefoil
appears to be adequate for stand maintenance making overseeding
unnecessary once a stand has been established.
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