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February, 1999

Diphenylether Herbicide Crop Response Study

by Donald E. Null, Regional Agronomy Specialist
University Outreach and Extension, NW Region

Situation: Three diphenylether herbicides are commonly used in Missouri herbicidal weed control programs in soybeans. The compounds are aciflurofen (Blazer/Status), lactofen (Cobra) and fomesafen (Flexstar/Reflex).

The diphenylether herbicides are extremely efficacious on the pigweed species. Since common waterhemp has become one of the most troublesome weeds in north Missouri, the diphenylether herbicides have been widely used to control it. Producers have shown considerable concern over their use, however. The diphenylether herbicides are contact-type, cell membrane disrupting compounds. Soybean response to the diphenylethers can best be characterized as "plant tissue burn". That is, soybean leaves respond to diphenylether applications by exhibiting mild to severe necrotic symptoms, depending upon the diphenylether herbicide used, environmental conditions, rate applied and tank mix partner. Producers commonly refer to "fried beans" as a response to diphenylether herbicide applications.

Many soybean producers are fearful that soybean yields are reduced due to the necrosis that accompanies the use of this class of chemistry. Research, under weed free conditions, needed to be conducted to determine the effect of diphenylether herbicides on soybean yield.

Objective: The objective of this study was to determine if soybean yields were adversely affected by applications of diphenylether herbicides. We hoped to be able to answer the questions relative to the differences in soybean yield response to two commonly used diphenylether herbicides; differences in soybean yield relative to diphenylether rate used; differences that application timing might have on soybean yields; differences that planting date might have upon soybean yield response to diphenylether herbicides; and differences that row width might have upon soybean yield response to the diphenylether herbicides.

Methods: A Split Block study, with six replications, was planned and planted during the springs of 1997 and 1998. Plot size was 6' wide by 30' long. A total of 36 treatments were used in the study. They included combinations of the following:

     1.   Lactofen or fomesafen at one-half and full labeled rates 
          with additives (additives were Sunnit II, 1% v/v and UAN,
          1% v/v).
     2.   Two herbicide application times.  One early and one late 
          (planned for V3 and R1 soybean growth stages).
     3.   One early and one late planting date (May 20 and June 28).
     4.   Two row widths (10" drilled and 36" wide rows).

The study was designed to be conducted without weed competition to the soybeans. Asgrow AG3701RR was selected as the variety of choice for the experiment. It was planted at the rate of 228,000 seeds per acre in 10" rows and at the rate of 196,000 seeds per acre in 36" wide rows. Soybeans were planted on May 20 to one-half of the plot area. The same variety, rate and row width were used to plant the other one-half of the plot area on June 28. The late plantings were no-tilled into a stale seedbed. A 6' wide, Tye Pasture Pleaser, no-till drill was used to plant the 10" wide rows and a John Deere 7300 vacuum planter was used to plant the 36" wide rows. Roundup at 1 quart per acre was broadcast applied with a field sprayer over the plot area on July 1 and July 31, 1998, to provide complete weed control.

The diphenylether herbicides were applied with a CO2 backpack sprayer delivering 20 gallons of spray solution per acre.

Even though the study was designed as a Split Block, treatments were controlled rather than completely randomized. In actuality, the study could best be described as four individual Randomized Complete Block studies. This allowed for RCB statistical analysis of the treatments. Plot layout was as follows:

Block 1  Early planted soybeans  Narrow rows  8 DPE treatments and 1
                                                       untreated
Block 2  Early planted soybeans  Wide rows    8 DPE treatments and 1
                                                       untreated
Block 3  Late planted soybeans   Narrow rows  8 DPE treatments and 1
                                                       untreated
Block 4  Late planted soybeans   Wide rows    8 DPE treatments and 1
                                                       untreated

Study Results: The data for all 36 treatments were collected and analyzed to provide a complete split plot factorial AOV table with an LSD significance level of 5%. There was a 9.9 bu/acre difference between the early and the late planting date. This was highly significant (LSD=4.1 bu/acre). For a second year, the value of narrow rows to soybean yield was higher with early planting than with late planting. This is contrary to what would have been expected. Figure 12 at right illustrates the crop yield response to row width and planting date. Crop yield for these "no DPE treatments" is expressed in percent of maximum untreated yield.

Figure 12
Figure 12

The maximum yield was 58.9 bu/acre for the early planted, drilled soybeans. The LSD at the 5% probability level was 6.1%.

The yields of late planted soybeans were more adversely affected by the lactofen treatments than by the fomesafen treatments. Figure 13 shows that, compared to the untreated check, both the one-half and full rates of lactofen adversely impacted soybean yields when it was applied to soybeans at either V3 or R1 growth stage in wide rowed soybeans. Only the R1 applied full rate of lactofen exhibited a significant yield decline in the late planted, drilled soybeans.

Figure 13
Figure 13 Effect of lactofen and fomesafin on late planted,
wide-rowed soybeans

Conclusion: We hoped to answer five questions with this research. The following is an attempt to do that based on the data produced from the two-year study.

Was there a difference in soybean yield response to the two commonly used diphenylether herbicides used in the study? The 1997 & 1998 experimental data each suggest that lactofen treatments had more of an adverse effect upon late planted soybean yields than fomesafen. There was no adverse crop response to any of the fomesafen applications. There was no significant adverse crop response to the lactofen or fomesafen applications made to early planted soybeans.

Was there a difference in soybean yield response to either one-half or full labeled rates of each of the two commonly used diphenylether herbicides? There was no significant difference in soybean yield between application rates for either of the diphenylether herbicides.

Did application timing have an effect upon soybean yield response to the diphenylether herbicides? We had anticipated that any adverse crop yield response would occur on the latest planted beans that were treated with a diphenylether at the R1 growth stage. This hypothesis was supported by the soybean yield response to the lactofen treatments in both 1997 & 1998.

Did planting date have an effect upon soybean yield response to the diphenylether herbicides? Both the 1997 and 1998 data suggest that there was no early planted crop yield response to the diphenylether herbicide treatments in the experiment. However, lactofen applications did provide an adverse soybean yield response to the late planted soybeans. In 1998, applications of the full labeled rate of lactofen to either the V3 or R1 stage soybeans adversely affected late planted soybean yields.

Did planting row width have an effect upon soybean yield response to the diphenylether herbicides? An analysis of both the 1997 & 1998 data suggests that late planted, wide rowed soybeans were more likely to be damaged by DPE applications than when the same treatments were applied to late planted, drilled soybeans.


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