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Bruce Burdick
1109 S. Birch St.
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Email: BurdickB@missouri.edu

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Food Grade Soybean Seed Size as Influence by Planting Date, Maturity Group and Plant Population
2002 Progress Report (12-27-02)

by
Donald E. Null, Regional Agronomy Specialist and Hundley-Whaley Research Farm Superintendent, University of Missouri
&
Steve Norberg, Ph.D., Morgan County Extension Director and Agronomy/Horticulture Agent, Colorado State University Cooperative Extension
&
Brian Thompson, Adult Farm Management Instructor,
Grand River Technical School, Chillicothe, MO 64601
&
Bruce Burdick, Research Associate, CAFNR Plant Science Unit

Situation: Larger seed size (fewer seeds/pound) is desirable in some of the contracts of higher value soybeans. Seed size may be impacted by soybean variety, maturity group, planting date and plant population or an interaction of these factors. Each of these factors was selected since they can potentially affect the number of viable seeds produced per pound of seed. A better understanding of these factors and their interactions on seed size would aid valued added producers in Missouri.

Objectives: Determine if decreasing plant population, decreasing maturity and delaying planting date will successfully increase seed size of food grade soybeans without large yield reductions over years.

Methods: The first year of the two-year study was conducted at the Hundley-Whaley Farm at Albany, Missouri. Dr. Norberg worked with Alex Stemme, Missouri Soybean Association, in securing four tofu varieties for the study (Table 1).

Variety Designation
Maturity Group
(MG)
Seeds/pound
Vinton 81
1.8
2150
Fontanelle 12-HC29
2.9
2420
Fontanelle 12-HC31
3.1
2020
Neco 53-3559A
3.9
2400
Table 1

Each soybean variety was planted at two planting dates and three populations in the study. Seed size, as a component of yield is not normally influenced by plant population. Since seed size is not greatly influenced by plant population, an ultra-low seed count providing an equivalent of 25,000 seeds/acre was used in the study to increase the likelihood of getting an increase in soybean seed size. (Table 2).

Planned Planting
Dates
Plant Populations
(seeds/acre)
May 10
25,000
June 3
87,500
 
150,000
Table 2

The study was designed as a Randomized Complete Block with split, split plot treatments, replicated five times. The whole plot was planting date, the split plot was soybean variety (maturity), and plant populations was the split-split plot. Planter row width was 30" wide. Plot size was 10' wide by 25' long.

The soybean seed was counted for each plot and then planted with a John Deere MaxEmerge planter equipped with cone planting units. Original seed size of the seed beans is in Table 1.

Seed bed preparation was accomplished by multiple disking of the plot area prior to the first planting. The second planting was made to a stale seed bed.

Weed control was difficult to obtain, especially for volunteer corn. The test site was part of the corn variety testing program in 2001, and corn borer pressure had resulted in very heavy lodging and ear yield loss. The weed control program consisted of the following:

  • PE on 5-28 of Canopy (6 oz/a) + Valor (3 oz/a) + Dual II Magnum (1.33 pt/a) for broad spectrum broadleaf and grass control
  • Post on 6-17 of Pursuit DG (1.4 oz/a) + Cobra (4 oz/a) + Fusion (6 oz/a) + Crop Oil Concentrate (1.25% v/v) + AMS (12 lb/100 gallon) for cocklebur, waterhemp and volunteer corn control
  • Post on 7-16 of Basagran (1 qt/a) + Select (10 oz/a) + Crop Oil Concentrate (1 qt/a) for new flush of cocklebur and volunteer corn control

The soybeans were also treated with Lorsban (1 qt/a) on July 30 for control of a light infestation of two-spotted spider mites.

Plant counts were taken on June 26 to determine established plants per acre. All plants in the second row of the four-row, twenty-five foot long plots were counted to determine established plant population per acre.

The soybeans were harvested with a MF8 plot combine on October 16. This harvesting date was too late for the early planted Vinton 81 variety because it suffered severe shattering prior to harvest. This had an adverse effect on both yield and seed size.

No lodging was evident at harvest time.

At harvest, seed samples were collected from all plots for determination of seed size. One ounce of clean seed (per seed sample) was counted for each harvested plot to determine seed size. Seed samples from the first and second replication were used to determine protein content. These samples were sent to Livestock Nutrition Laboratory Services in Columbia, Mo. for determination of protein by the combustion method.

Results and Discussion: The date of planting for the soybeans in this study varied from the dates planned due to wet weather conditions in the spring. The planned and actual planting dates are in Table 3.

Planned Planting
Dates
Actual Planting
Dates
May 10
May 28
June 3
June 14
Table 3

The mid-June planting date resulted in a better stand of soybeans, increased soybean yield, larger soybean seed and lower protein content than beans planted in late May (Table 4).

Planting Date
Plants/acre
established
Yield
(bu/acre)
Seeds/
pound
Protein
May 28
41223
31.4
2191
39.1
June 14
47674
41.7
2072
38.1
Table 4

Seedling establishment was less than anticipated for both planting dates, ranging from 41 to 65 percent of seed planted. The mid-June planting date provided better overall stand of established plants than the late-May planting (Table 5 & 6).

The lowest planting rate of 25,000 seeds per acre resulted in the lowest soybean stand, the lowest soybean yield and the largest soybean seed size. The data does suggest that seed size can be influenced by the stand of soybeans obtained, however the reduction in overall seed yield due to a reduced stand of soybeans is quite substantial. A soybean stand of 46,507 plants per acre provided soybean yields that were statistically insignificant from stands that were higher (Table 5).

Protein content of the soybean seed was only slightly affected by stand establishment, and the trend was for protein content to increase with planting rate and stand establishment (Table 5).

Planted Population
Plants/acre
established
Yield
(bu/acre)
Seeds/
pound
Protein
25,000
14020
29.0
2070
38.28
87,500
46507
40.1
2158
38.64
150,000
72819
40.6
2168
38.90
LSD (P=0.1)
1.5
39
0.56
Table 5

Soybean Variety
Established Seedlings (plants/acre & percent established)
Planting Rate
May 28 Planting
June 14 Planting
Plant count
Percent Established
Plant count
Percent Established
Vinton 81
25,000
11,709
47%
14,916
60%
Vinton 81
87,500
40,983
47%
40,565
46%
Vinton 81
150,000
66,354
44%
77,785
52%
Fontanelle 12-HC29
25,000
10,594
42%
14,776
59%
Fontanelle 12-HC29
87,500
35,965
41%
42,099
48%
Fontanelle 12-HC29
150,000
62,312
42%
75,415
50%
Fontanelle 12-HC31
25,000
13,661
55%
16,310
65%
Fontanelle 12-HC31
87,500
53,111
61%
56,039
64%
Fontanelle 12-HC31
150,000
69,979
47%
83,082
55%
Neco 53-3559A
25,000
14,716
59%
15,473
62%
Neco 53-3559A
87,500
50,184
57%
53,111
61%
Neco 53-3559A
150,000
65,100
43%
82,525
55%
Average
  
49%
 
56%
Table 6

Fontanelle 12-HC31 and Neco 53-3559A tended to provide a higher percentage of established plants than Vinton 81 and Fontanelle 12-HC29 (Table 6).

The later-than-planned planting dates actually worked to the advantage of soybean yield this year (2002). When averaged over all planting rates and varieties, the later planted soybeans yielded 10.3 bu/acre more than the early planted soybeans. The maturity group 3 soybean varieties provided higher yields than the maturity group 1 and 2 soybean varieties. Yield by variety and planting date are shown in Tables 7 & 8.

Soybean Variety
Soybean Yield (bu/acre)
May 28 Planting Date
June 14 Planting Date
Vinton 81
25.3
34.4
Fontanelle 12-HC29
28.0
40.6
Fontanelle 12-HC31
37.5
44.8
Neco 53-3559A
35.0
47.1
Mean of four varieties
31.4
41.7
Table 7

Soybean variety
Soybean Yield (bushels/acre)
Planting Rate
Planted May 28
Planted June 14
Mean Yield
Vinton 81
25,000
19.6
24.3
21.9
Vinton 81
87,500
27.3
38.3
32.8
Vinton 81
150,000
29.0
40.4
34.7
Fontanelle 12-HC29
25,000
18.1
32.3
25.2
Fontanelle 12-HC29
87,500
31.1
44.9
38.0
Fontanelle 12-HC29
150,000
34.7
44.5
39.6
Fontanelle 12-HC31
25,000
29.8
37.4
33.6
Fontanelle 12-HC31
87,500
42.9
48.2
45.6
Fontanelle 12-HC31
150,000
39.8
48.8
44.3
Neco 53-3559A
25,000
29.5
41.0
35.2
Neco 53-3559A
87,500
37.1
51.1
44.1
Neco 53-3559A
150,000
38.4
49.3
43.9
LSD (P=0.1)
4.2
3.0
Table 8

Seed size was affected by planting rate (Table 9). There was a statistically significant difference in seed size between the soybeans planted at 25,000 seeds per acre and those planted at 150,000 seeds per acre. This was regardless of planting date or variety. However, the difference in Vinton 81 soybean seed size in relation to plant population was directly opposite the other three soybean varieties. Fontanelle 12-HC29, Fontanelle 12-HC31 and Neco 53-3559A seed decreased in size as plant population increased. The only explanation for this is that the Vinton 81 soybeans had suffered moderate to severe shattering prior to harvest, and it is likely that the pods with the largest seed size would be the first to shatter.

Soybean Variety
Seed Size (seeds/pound)
Planting Rate
May 28 Planting Date
June 14 Planting Date
Vinton 81
25,000
2310 ABC
2105 EFGH
Vinton 81
87,500
2186 DE
2071 FGHI
Vinton 81
150,000
2177 EF
1917 K
Fontanelle 12-HC29
25,000
2204 CDE
2120 EFGH
Fontanelle 12-HC29
87,500
2371 AB
2289 BCD
Fontanelle 12-HC29
150,000
2416 A
2319 AB
Fontanelle 12-HC31
25,000
2017 HIJK
1944 JK
Fontanelle 12-HC31
87,500
2126 EFGH
2029 HIJ
Fontanelle 12-HC31
150,000
2189 DE
2111 EFGH
Neco 53-3559A
25,000
1950 JK
1911 K
Neco 53-3559A
87,500
2198 DE
1993 IJK
Neco 53-3559A
150,000
2150 EFG
2065 GHI
LSD (P=0.1)
110
Table 9

Soybean variety
Soybean Protein (percent)
Planting Rate
Planted May 28
Planted June 14
Mean
Vinton 81
25,000
40.1
39.2
39.7
Vinton 81
87,500
41.0
40.2
40.6
Vinton 81
150,000
39.9
40.8
40.3
Fontanelle 12-HC29
25,000
37.8
36.5
37.1
Fontanelle 12-HC29
87,500
37.9
36.2
37.0
Fontanelle 12-HC29
150,000
38.4
36.6
37.5
Fontanelle 12-HC31
25,000
38.4
38.2
38.3
Fontanelle 12-HC31
87,500
39.3
38.7
38.9
Fontanelle 12-HC31
150,000
39.4
38.5
39.0
Neco 53-3559A
25,000
39.3
36.9
38.1
Neco 53-3559A
87,500
38.5
37.4
38.0
Neco 53-3559A
150,000
39.3
38.3
38.8
LSD (P=0.1)
1.7
1.2
Table 10

Variety
Plants/acre established
Yield (bu/acre)
Seeds/
pound
Protein
Vinton 81
42052
29.8
2128
40.2
Fontanelle 12-HC29
40194
34.3
2286
37.2
Fontanelle 12-HC31
48697
41.2
2069
38.7
Neco 53-3559A
46852
41.1
2045
38.3
LSD (P=0.1)
2345
1.7
45
0.65
Table 11

Protein content of the seed was influenced by soybean variety. Vinton 81 was the containing the most protein while Fontannell12-HC29 was the variety containing the least amount of protein. There was a trend for the earlier planted soybeans to contain higher protein than the later planted soybeans. Plant population had little to no effect on protein content (Table 10).

Fontanelle 12-HC31 and Neco 53-3559A provided better stands of higher yielding, larger seeded soybeans than Vinton 81 or Fontanelle 12-HC29 soybeans (Table 11).

Summary: Higher soybean yields were achieved from the June 14 planting date this year. Soybeans planted two weeks earlier yielded 10.3 bu/acre less than the later planted beans. Soybean yield varied by variety. Maturity group appeared to be a factor in soybean yield. The earlier maturing soybeans yielded less than the later maturing soybeans. Very low plant populations (14,000 plants per acre) had an adverse effect on soybean yield. There was no significant improvement in yield at soybean plant populations over 46,500 plants per acre.

The late planted soybeans yielded the largest seed size this year. Seed size was also impacted by plant population, but it took very low plant populations to yield a larger bean. The variety of soybean used in the study made a significant difference in seed size. Neco 53-3559A produced the largest bean and Fontanelle 12-HC29 produced the smallest bean. The two maturity group 3 soybean varieties produced seed of larger size than the two varieties in the study that matured earlier.

Soybean protein content ranged from 37 to 40% and varied by variety in the study. Vinton 81 contained the highest protein content and Fontanelle 12-HC29 contained the lowest protein. There was no trend apparent relative to protein content in relation to maturity group The earlier planting date yielded soybeans with 1% higher protein than the earlier planting date. Protein content was lowest with the lowest plant population, and highest with the highest plant population.


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